JHDN-5 antiserum induces mitochondrial oxidative stress in HepaRG cells. (A to C) Confocal images of HepaRG cells treated for 2 h with (A) mouse IgG (1:100, negative control), (B) JHDN-5 antiserum (1:100), or (C) TBHP (150 µM, positive control) followed by MitoSOX Red. (D) Mitochondrial oxidative stress levels were higher after treatments with JHDN-5 IgG or TBHP than after treatment with mouse IgG; ***, P < 0.001. JHDN-5-induced mitochondrial oxidative stress was not significantly different from that induced by TBHP. Separate wells were treated with (E) JHDN-5 IgG alone, pretreated with (F) NAC, 200 µM, 1 h before JHDN-5 IgG, or (G) treated with MnTBAP, 100 µM, for the last 30 min of antibody treatment followed by MitoSOX Red reagent. (H) JHDN-5 IgG-induced oxidative stress was significantly lowered by MnTBAP; *, P < 0.05. (McCarthy et al., 2019)
TFA-S100 induces hepatitis in BALB/c mice. BALB/c mice were immunized sc with CFA (A), S100/CFA (B), or TFA-S100/CFA (C) and liver samples collected 3 weeks following initial immunization according to the Materials and methods. Liver sections were stained with H&E and assessed for the degree of inflammation and injury according to a standard scoring system (magnification 100×). (Njoku, et al., 2009)
